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KMID : 0368419910340010031
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Journal of Plant Biology
1991 Volume.34 No. 1 p.31 ~ p.35
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Changes of Amino Acid Content of Cultured Tobacco Cells by Association Culture with Nostoc muscorum
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Abstract
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Induction of symbiosis between N. muscorum and cultured tobacco cells associately cultured on nitrogen-free medium and effects of polyamines on culture condition were carried out. Analysis of amino acid composition in associate cultures showed increase in total amino acid amounts than single culture of cultured tobacco cells and methionine was markly increased in associative culture on N-free medium treated with 1 mM spermine. These results indicated that the composition of amino acids increased effectively in associative cultures by nitrogen fixation of N. muscorum.
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